Platelet-rich plasma preparation using three different centrifugation methods: A comparative study

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Abstract

Background
Platelet-rich plasma (PRP) is a platelet concentrate contained in a small volume of plasma, it has been a promising option in the last decade to treat different dermatology diseases, such as alopecia, skin ulcers, melisma, and burns due to the high concentration of growth factors that stimulate tissue repair and regeneration. The current study aims to compare the yield of platelets comparing different centrifugation methods, which are double-spin high-centrifugation force, double-spin low-centrifugation force, and single-spin system correlating each with the growth-factor concentration.
Patients and methods
This study was performed on 30 healthy individuals (12 males and 18 females), their age ranged from 17 to 49 years old. From each individual, 20 ml of venous blood was collected for both basal blood-cell count and for PRP preparation. The PRP samples were prepared by differential centrifugation methods through one of three different procedures using the laboratory centrifuge (Eppendorf 5810 R). Growth factors, including vascular endothelial growth factor, platelet-derived growth factor, and matrix metalloprotein-9, were measured in PRP samples using enzyme-linked immunosorbent assay technique.
Results
The current study showed that the double-spin low-centrifugation method increased the platelet count by 140% than the baseline value, with significant fold rise than in the single-spin and the double-spin high-centrifugation method, meanwhile, there is a statistically significant increase in the corresponding growth factors.
Conclusions
Double-spin low-centrifugation method and single-spin centrifugation provide better platelet yielding with efficient growth-factor concentrations.

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